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DNA Oligonucleotides
All single-stranded and duplexed DNA sequences are produced with high coupling efficiencies, resulting in high-quality DNA products. Our specialized platforms allow us to deliver primers for PCR, dual-labelled probes for qPCR, indexed adapters and fusion primers for sequencing, and a variety of custom products.
Options:
- Single-Stranded DNA – Shipped dry, or resuspended to your specifications.
- Normalized Oligo – Custom normalized oligos up to 10 grams
- RxnReady™ Primer Pools – 2 oligos premixed in a single tube. Shipped dry, or resuspended to your specifications.
Duplexed DNA – 2 oligos, annealed and delivered in a single tube. Shipped dr
PROBES
PrimeTime™ qPCR Probes
PrimeTime qPCR Probes are 5′ nuclease probes, available with an assortment of reporter-dye combinations that are compatible with common qPCR instruments and that include several license-free combinations.
Options:
• Express
• Standard
• Complex
• Mini/Eco
Affinity Plus™ qPCR Probes
Use Affinity Plus™ qPCR Probes for enhanced discrimination of thermodynamically similar samples such as single nucleotide polymorphisms and transcript variants. The Affinity Plus bases used in these qPCR probes include up to 6 locked nucleic acid monomers (see the technology page, Locked nucleic acids, for more information). When incorporated into a probe, locked nucleic acids impart heightened structural stability, leading to increased hybridization melt temperature (Tm).
Options: Mini and Standard
Molecular beacon probes
Molecular beacon probes are dual-labeled probes that form a quenched, stem-loop structure in native state and fluoresce upon hybridization to the target nucleotide sequence. Applications include real-time and endpoint PCR, SNP detection, and multiplex amplification.